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thaliana seedlings were exposed to ITC for 10 days while growing vertically on square plates containing solid in vitro medium as described above

The constructs were transformed into TOP10 cells, and empty plasmid or plasmid containing the guide-RNA was isolated and transiently transfected into the AuxB1 or CHO R C5 cells using the Lipofectamine 2000 kit (Invitrogen)

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